DeNovoMAGIC™

The cannabis haploid genome is comprised of 735 million base pairs (Mbp). It is highly heterozygous, which means most of the regions (loci) along the genome carry two dissimilar sequences (alleles). Most assembly tools generate a synthetic (haploid) genome, resulting in a sequence for cannabis that is typically a mix (chimera) of both alleles.

NRGene’s DeNovoMAGIC™ assembly tool takes different approach - by accurately phasing the two alleles, we are able to deliver a true representation of the genome

ASSEMBLY STATISTICS FOR CANNABIS GENOMES

UNPHASED

PARAMETER:Results Range
ASSEMBLY SIZE0.795 -0.812 (Gbp)
Gaps %2.2- 2.8 (%)
N5012.3-18.6 (Mbp)
N50 #SEQUENCES14-19
COMPLETE BUSCOS95.4-97.2 (%)
COMPLETE AND SINGLE-COPY BUSCOS91.6-94.1 (%)

PHASED

PARAMETER:Results Range
ASSEMBLY SIZE1.35-1.46 (Gbp)
Gaps %2.2-2.8 (%)
N502.5-2.8 (Mbp)
N50 #SEQUENCES120-134
COMPLETE BUSCOS97.6-97.9 (%)
COMPLETE AND SINGLE-COPY BUSCOS11.4-28.7 (%)
COMPLETE AND DUPLICATED BUSCOS69.2-86.2 (%)

AN ALL-IN-ONE PACKAGE THAT TAKES YOU FROM PLANT TISSUE TO GENOME ASSEMBLY IN RECORD TIME.

SERVICE PACKAGE INCLUDES

  • High quality denovo genome assembly of large scaffolds
  • Construction of Pseudo-chromosomes

Let’s get started!