// DeNovoMAGIC™- Hybrid
The ultimate tool for reference genome assembly:
Receive the highest genome assembly quality in a record time. DeNovoMAGIC™ has already been successfully used by hundreds of customers around the globe, building the most challenging reference genomes. Now, with the availability of an accurate long read data our quality got even better.
The newest version of DenovoMAGIC™ employs a real hybrid assembly to benefit the data quality and unique genome coverage of each: short Illumina reads and long PacBio HiFi reads.
Outstanding Results
| CROP | CANNABIS | SWEETCANE | SWEET BASIL | CITRUS |
|---|---|---|---|---|
| PLOIDY | Diploid | Tetraploid | Tetraploid | Diploid |
| ZYGOSITY | Heterozygote | Heterozygote | Homozygote | Heterozygote |
| HAPLOID GENOME SIZE (Gbp) | 0.8 | 3.4 | 2.4 | 0.4 |
| TOTAL ASSEMBLY SIZE (Gbp) | 1.6 | 7.1 | 2.6 | 0.63 |
| CONIG N5O (L5O) (Mbp) | 1.681 (197) | 1.53 (853) | 13.1 (57) | 2.36 (57) |
| COMPLETE BUSCO GENES | 98.3% | 97% | 97.5% | 98.2% |
| SHORT READS APPROXIMATE COVERAGE (PE250X2) | x35 | x35 | x35 | x35 |
| LONG READS APPROXIMATE COVERAGE (HiFi) | x20 | x20 | x20 | x20 |
Advantages
DeNovoMAGIC™ – Hybrid provides you with best-in-class genome assembly results
DNA-to-Assembly in record delivery time and low error rate – less than 1 in 3Mbp!*
Proven technology – published in peerreviewed papers, and widely used by big corporations
A turn-key solution from DNA to DeNovo genome assembly regardless of complexity and ploidy
Benefiting from complementary attributes of both platforms to reach a gapless assembly
Each contig accurately represents a single allele/ homeolog/ paralog sequence. The output represents all unique sequences present
Ordering to pseudo chromosomes using an existing reference
*Avni, R. et al. 2017. Wild emmer genome architecture and diversity elucidate wheat evolution and domestication. Science, 357(6346)
How it Works
An extensive yet simple tool for analyzing, storing and mining unlimited volumes of genomic data
DeNovoMAGIC delivers the most progressive and novel ability. The only method to truly discover the entire content of the genome!
Related Videos
Webinar:
Accelerating Crop Research with High-Quality Affordable Genome Assemblies
“NRGene’s New DeNovo Product Portfolio”
Building a Dolphin Genome Assembly
Frequently Asked Questions
For every diverged homolog or homeolog sequence (originating from heterozygosity or polyploidy respectively) there will be two different scaffolds in the delivered assembly, correctly capturing the actual genome sequence.
Results can be delivered in 4 months or less from sequence data availability
Yes, when provided with an existing physical or genetic map, we can order scaffolds to pseudo-molecules. If no such map is available, we can generate one for you. See our TraitMAGIC offer for more details on the creation of a genetic map.
