// DeNovoMAX™
The optimal solution for homozygous genome assembly
DeNovoMAX is an innovative solution based on the underlying technology of our renowned DeNovoMAGIC assembly. Leveraging NRGene’s vast experience and latest sequencing technologies, we have developed a leaner setup
to optimize our whole genome assembly solution to meet your budget needs while maintaining a very high-quality standard. This end-to-end solution is now available for
homozygote genomes in various ploidy and sizes.
Now we can incorporate Pac-Bio’s
long-reads technology in our assemblies.
Outstanding Results
| CROP | PEPPER (Diploid) | SUNFLOWER (Diploid) | SOY (Diploid) | COTTON (Tetraploid) | SWEET CORN (Diploid) | BREAD WHEAT (Hexaploid) |
|---|---|---|---|---|---|---|
| TOTAL ASSEMBLY SIZE | 3.26 Gbp | 3.32 Gbp | 1.01 Mbp | 2.47 Gbp | 2.36 Gbp | 14.58 Gbp |
| SCAFFOLD N50 (# OF SCAFFOLDS) | 35.17 Mbp (25) | 6.69 Mbp (149) | 11.49 Mbp (29) | 17.63 Mbp (43) | 10.63 Mbp (66) | 29.13 Mbp (129) |
| SCAFFOLD N90 (# OF SCAFFOLDS) | 1.36 Mbp (211) | 1.01 Mbp (571) | 1.54 Mbp (114) | 3.56 Mbp (154) | 1.96 Mbp (258) | 4.03 Mbp (619) |
| UNFILLED GAPS (%N) | 1.4% | 0.8% | 3.4% | 1.4% | 0.5 | 0.9% |
| COMPLETE BUSCO GENES | 95.6% | 91.0% | 98.3% | 96.1% | 97.1% | 98.3% |
| SHORT READS COVERAGE | 95X | 95X | 95X | 95X | 95X | 95X |
These are just examples, we can cover many more organisms!
Advantages
High standards without compromising on accuracy
Unprecedented price offering thanks to a lean library recipe
A turnkey solution from DNA to De Novo assembly
DNA to Assembly in record delivery time
Incorporate PacBio’s long reads in your assembly
How it Works
DeNovoMAX offers a lean libraries recipe for main commercial genomes to form a setup with minimal compromise in quality, and is offered in unprecedented affordable price.
DATA RECIPE
| LIBRARY TYPE | INSERT SIZE | READS LENGTH | COVERAGE |
|---|---|---|---|
| PCR-FREE SHOTGUN PAIR-END | 470 bp | 250X | 45X |
| NEXTERA MATE-PAIR | 2-4 kbp | 150X | 25X |
| 10X CHRMOIUM | N/A | 150X | 25X |
| TOTAL | 95X |
Related Videos
“NRGene’s New DeNovo Product Portfolio”
Webinar
Accelerating Crop Research with High-Quality Affordable Genome Assemblies
Frequently Asked Questions
- DeNovoMAX is designed to provide cost-effective and reduced cost solution for homozygote genomes. After several months of research and trials, NRGene has managed to calibrate a slimmer sequencing recipe with only half of the genomic libraries and less than half of the total sequencing coverage, while still retaining high-quality, accurate and full assemblies. The great reduction in sequencing amount and costs is rolled straight to the customers, with reduced prices of up to 70%! Comparing to previous prices of DeNovoMAGIC 3.
NRGene PanMAGIC is a full, high-qualilty package for pangenomic comparisons. In PanMAGIC both genomes made by DeNovoMAX and DeNovoMAGIC may be included to produce a complete set of genome-to-genome pairwise mapping and an extensive transcripts mapping (alignment based annotation) and comparisons between a single pivot and each annotated genome (identifying Presence-absence variations, Copy-number variations and Structural variations).
DeNovoMAX is available for most major inbred crops and ultimately any homozygous genome may be assembled. Non-inbreds varieties and/or heterozygous genomes are not applicable for DeNovoMAX due to the higher complexity of the genome and required analysis. Nonetheless DeNovoMAGIC 3 is still available for these genomes providing the highest standard of assembly and featuring proven results- published in high-impact magazines.
Yes, NRGene has developed a new approach to integrate long-reads data (PacBio SMRT Sequell™), the long-reads can be used to improve contiguity and fill gaps within scaffolds and An example of this approach is featured in the recent Science magazine publication of the Poppy genome: https://science.sciencemag.org/content/362/6412/343
