// DeNovoMAXTM

The optimal solution for homozygous genome assembly

DeNovoMAX is an innovative solution based on the underlying technology of our renowned DeNovoMAGIC assembly. Leveraging NRGene’s vast experience and latest sequencing technologies, we have developed a leaner setup
to optimize our whole genome assembly solution to meet your budget needs while maintaining a very high-quality standard. This end-to-end solution is now available for
homozygote genomes in various ploidy and sizes.

Now we can incorporate Pac-Bio’s
long-reads technology in our assemblies.

Results and Pricing samples

New product introduction prices – over 50% price reduction from original 210X coverage

CROPPEPPER (Diploid)SUNFLOWER (Diploid)SOY (Diploid)COTTON (Tetraploid)SWEET CORN (Diploid)BREAD WHEAT (Hexaploid)
TOTAL ASSEMBLY SIZE3.26 Gbp3.32 Gbp1.01 Mbp2.47 Gbp2.36 Gbp14.58 Gbp
SCAFFOLD N50
(# OF SCAFFOLDS)
35.17 Mbp (25)6.69 Mbp (149)11.49 Mbp (29)17.63 Mbp (43)10.63 Mbp (66)29.13 Mbp (129)
SCAFFOLD N90
(# OF SCAFFOLDS)
1.36 Mbp (211)1.01 Mbp (571)1.54 Mbp (114)3.56 Mbp (154)1.96 Mbp (258)4.03 Mbp (619)
UNFILLED GAPS (%N)1.4%0.8%3.4%1.4%0.50.9%
COMPLETE BUSCO GENES95.6%91.0%98.3%96.1%97.1%98.3%
SHORT READS COVERAGE95X95X95X95X95X95X
PRICE$47K$47K$30K$39K$39K$145K

These are just examples, we can cover many more organisms!

Advantages

ACCURATE

High standards without compromising on accuracy

COMPETITIVE PRICING

Unprecedented price offering thanks to a lean library recipe

FULL SERVICE

A turnkey solution from DNA to De Novo assembly

FAST DELIVERY

DNA to Assembly in record delivery time

OPTIONAL ADD-ON

Incorporate PacBio’s long reads in your assembly

How it Works

DeNovoMAX offers a lean libraries recipe for main commercial genomes to form a setup with minimal compromise in quality, and is offered in unprecedented affordable price.

DATA RECIPE
LIBRARY TIPEINSERT SIZEREADS LENGTHCOVERAGE
PCR-FREE SHOTGUN PAIR-END470 bp250X45X
NEXTERA MATE-PAIR2-4 kbp150X25X
10X CHRMOIUMN/A150X25X
TOTAL95X

Related Videos

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“NRGene’s New DeNovo Product Portfolio”

 

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Accelerating Crop Research with High-Quality Affordable Genome Assemblies

 

Frequently Asked Questions

How DeNovoMAX is different from DeNovoMAGIC?
  1. DeNovoMAX is designed to provide cost-effective and reduced cost solution for homozygote genomes. After several months of research and trials, NRGene has managed to calibrate a slimmer sequencing recipe with only half of the genomic libraries and less than half of the total sequencing coverage, while still retaining high-quality, accurate and full assemblies. The great reduction in sequencing amount and costs is rolled straight to the customers, with reduced prices of up to 70%! Comparing to previous prices of DeNovoMAGIC 3.
I want to compare between several genomes. is that available for both DeNovoMAX and DeNovoMAGIC?

NRGene PanMAGIC is a full, high-qualilty package for pangenomic comparisons. In PanMAGIC both genomes made by DeNovoMAX and DeNovoMAGIC may be included to produce a complete set of genome-to-genome pairwise mapping and an extensive transcripts mapping (alignment based annotation) and comparisons between a single pivot and each annotated genome (identifying Presence-absence variations, Copy-number variations and Structural variations).

Is the offer of DeNovoMAX limited to specific organisms?

DeNovoMAX is available for most major inbred crops and ultimately any homozygous genome may be assembled. Non-inbreds varieties and/or heterozygous genomes are not applicable for DeNovoMAX due to the higher complexity of the genome and required analysis. Nonetheless DeNovoMAGIC 3 is still available for these genomes providing the highest standard of assembly and featuring proven results- published in high-impact magazines.

Can you integrate long-reads data in the assembly?

Yes, NRGene has developed a new approach to integrate long-reads data (PacBio SMRT Sequell™), the long-reads can be used to improve contiguity and fill gaps within scaffolds and An example of this approach is featured in the recent Science magazine publication of the Poppy genome: https://science.sciencemag.org/content/362/6412/343