Pave the way to smarter breeding by unraveling the causation elements in your population genomics
Quickly discover the phenotype-genotype correlations in any organism with NRGene’s complete trait-mapping package.
We’ll generate a high-resolution recombination map which
is used to reveal the complete segregation pattern in each progeny. Our special skim-sequencing derived analysis brings a new level of detail to genomic analysis and unlocks the secrets of your population-genomics.
TraitMAGIC – Explained
Outstanding Results
REAL CASE STUDY 1 (HOMOZYGOTE):
241 significant loci affecting 129 fruit quality traits were identified by converting over 58,000 markers to 3,663 haplotype bins in a melon RIL population*
* Galpaz, N. et al. 2018. Deciphering Genetic Factors that Determine Melon Fruit‐Quality Traits Using RNA‐Seq‐Based High‐Resolution QTL and eQTL Mapping. The Plant Journal.
REAL CASE STUDY 2 (HETEROZYGOTE):
A phased haplotype approach (TraitMAGIC) was found superior to a consensus marker-based approach even when applied to <25% of a heterozygote F1 population.
| METHOD | # OF PROGENY ANALYZED | # OF LOCI IDENTIFIED | PREDICTION ACCURACY* |
|---|---|---|---|
| NRGENE | 200 | >8 | 70% |
| ALTERNATE | >800 | 1 | 0% |
* percentage of progeny whose phenotype can be predicted with high accuracy based on the results.
Advantages
Translate the most complex trait genetics to a simple and accurate prediction
We generate high-density marker maps for producing extremely accurate trait mapping, even in the most complex genomes
Proven ability to identify, and correctly phase and map sequences even in complex heterozygote cases
We provide sequencing, marker discovery, genotyping, and trait mapping.Send us DNA and phenotype data and we will do the rest
How it Works
AN EXTENSIVE YET SIMPLE TOOL FOR ANALYZING, STORING AND MINING UNLIMITED VOLUMES OF GENOMIC DATA
Related Videos
“NRGene’s webinar – Finding the needles in your haystack”
May 2019
“Ultra-High Resolution QTL Mapping of Fruit Quality Traits in Melon”
Speaker: Dr. Nurit Katzir
Agricultural Research Organization,
Newe Ya’ar Research Center
Frequently Asked Questions
It means that the two parental contributions of a segregating population are completely separated and parental meiotic components for each progeny are fully reconstructed.
Two unique features provide superior mapping results: 1. Phasing: it could be that each parent is heterozygous for different QTLs and only a phased map enables the detection of these. 2. Haplotype bins are used and not single markers, allowing for powerful statistics at the maximal true resolution (improving both specificity and sensitivity compared with using single markers).
Melon RIL pupulation: https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.13838
Sesame F12 population: https://doi.org/10.3390/genes11101221
